Production of human recombinant collagen in the milk of transgenic animals
OwnerRichard A. Berginventor
Life of this patent
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Abstract
Production of human procollagen or collagen in cells which ordinarily do not produce these molecules is effected by constructing expression systems compatible with mammary glands of non-human mammals. For example, expression systems can be microinjected into fertilized oocytes and reimplanted in foster mothers and carried to term in order to obtain transgenic non-human mammals capable of producing milk containing recombinant human procollagen or collagen. Human procollagen or collagen produced in this manner can be made of a single collagen type uncontaminated by other human or non-human collagens.
Claims12
A method for preparing human collagen or human procollagen, which method comprises:
- isolating milk from a non human mammal whose genome comprises an expression system comprising a nucleotide sequence encoding a human collagen or human procollagen operably linked to a promoter that functions in milk protein-secreting epithelial cells of the mammal, wherein the collagen or procollagen is expressed and secreted into the milk of the mammal;
- and
- recovering the procollagen or collagen from the milk.
An expression system for production of human collagen or human procollagen in milk which expression system comprises a nucleotide sequence encoding the collagen or procollagen operably linked to a promoter that functions in milk protein-secreting epithelial cells of mammary glands.
A polynucleotide comprising a sequence encoding human collagen or human procollagen operably linked to a promoter that functions in milk protein-secreting epithelial cells of mammary glands.
Milk made by isolating said milk from a non human mammal whose genome comprises an expression system comprising a nucleotide sequence encoding a human collagen or human procollagen operably linked to a promoter that functions in milk protein-secreting epithelial cells of the mammal, wherein the collagen or procollagen is expressed and secreted into the milk of the mammal.
Dependencies are inferred from the claim text.