Escape mutants of newcastle disease virus as marker vaccines
AssigneeWyeth
InventorsHarmen J. Geerligs, Ian Hamer Brown, Dennis John Alexander, Michael Sinclair Collins
Life of this patent
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Abstract
A vaccine against Newcastle Disease contains one or more mutant immunogens of the NDW strain. The mutant immunogen lacks the antigenic binding site on the F glycoprotein which is recognized by the monoclonal antibody mAb 54. Reagent kits and assay methods help to distinguish vaccinated members of a poultry flock from those that may have been infected with wild-type Newcastle Disease virus.
Claims19
A vaccine against Newcastle Disease virus, comprising about 100-109 EID50 of a mutant immunogen from the NDW strain of the Newcastle Disease virus, wherein said mutant immunogen lacks the antigenic binding site on the F glycoprotein recognized by the monoclonal antibody designated mAb 54.
An immunogen of Newcastle Disease virus having the immunogenic characteristics of the strain deposited with the CNCM under accession number # I-2928.
A method of protecting a poultry animal from Newcastle Disease, which comprises administering to said animal about 100-109 EID50 of a mutant immunogen from the NDW strain of the Newcastle Disease virus, wherein said mutant immunogen lacks the antigenic binding site on the F glycoprotein recognized by the monoclonal antibody mAb 54.
A method of generating a Newcastle Disease virus mutant immunogen useful in a vaccine against Newcastle Disease, which comprises growing Newcastle Disease virus in the presence of the monoclonal antibody designated mAb 54, such that said mutant immunogen develops and grows in the presence of said monoclonal antibody and is not neutralized by said monoclonal antibody.
The method of claim 11, wherein said Newcastle Disease virus is the NDW strain.
The method of claim 12, wherein said virus is grown in embryonated poultry eggs.
A mutant immunogen of Newcastle Disease virus wherein the F glycoprotein on said virus is derived from the partial nucleotide sequence shown in Seq. ID No.1, and further wherein said glycoprotein lacks the binding site for monoclonal antibody designated mAb 54.
A mutant immunogen having the amino acid sequence shown in Seq. ID No.2.
An immunogen of Newcastle Disease virus having the immunogenic characteristics of the mutant immunogen with the amino acid sequence shown in Seq. ID No. 2.
A mutant immunogen of Newcastle Disease virus in which the amino acid serine is replaced by arginine at position 157.
An assay kit for detecting inoculation via a mutant immunogen against Newcastle Disease, comprising a standard NDV antigen and an illuminating antibody, wherein said illuminating antibody binds to said antigen in inoculated sera, but does not bind to said antigen in uninoculated sera.
Dependencies are inferred from the claim text.