Inhibitors of apoptosis proteins in plants
AssigneeE. I. Du Pont de Nemours and Company
InventorsRebecca E. Cahoon, Perry G. Caimi, Theodore M. Klein, Michele Morgante, Hajime Sakai, Jennie Bih-Jien Shen, Scott V. Tingey
Life of this patent
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Abstract
This invention relates to an isolated nucleic acid fragment encoding an apoptosis inhibitory protein. The invention also relates to the construction of a chimeric gene encoding all or a portion of the apoptosis inhibitory protein, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the apoptosis inhibitory protein in a transformed host cell.
Claims27
An isolated nucleic acid fragment encoding all or a substantial portion of a DAD1 protein comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4 and 6;
- (b) an isolated nucleic acid fragment that is substantially similar to an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4 and 6;
- and
- (c) an isolated nucleic acid fragment that is complementary to (a) or (b).
A DAD1 polypeptide comprising all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:2, 4 and 6.
An isolated nucleic acid fragment encoding all or a substantial portion of an AAC-11 protein comprising a member selected from the group consisting of:
- (a) an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:8, 10 and 12;
- (b) an isolated nucleic acid fragment that is substantially similar to an isolated nucleic acid fragment encoding all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:8, 10 and 12;
- and
- (c) an isolated nucleic acid-fragment that is complementary to (a) or (b).
An AAC-11 polypeptide comprising all or a substantial portion of the amino acid sequence set forth in a member selected from the group consisting of SEQ ID NO:8, 10 and 12.
A method of obtaining a nucleic acid fragment encoding a substantial portion of an amino acid sequence encoding an apoptosis inhibitory protein comprising:
- (a) synthesizing an oligonucleotide primer corresponding to a portion of the sequence set forth in any of SEQ ID NOs:1, 3, 5, 7, 9 and 11;
- and
- (b) amplifying a cDNA insert present in a cloning vector using the oligonucleotide primer of step (a) and a primer representing sequences of the cloning vector
- wherein the amplified nucleic acid fragment encodes a substantial portion of an amino acid sequence encoding an apoptosis inhibitory protein.
Dependencies are inferred from the claim text.