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US 2004/0111765 A1Published application
Production of urokinase in plant-based expression systems
OwnerKaren K. Oishi, Da-Feng Zhouinventors
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OwnerKaren K. Oishi, Da-Feng Zhouinventors
Estimating the term…
The invention relates to the production of biologically active recombinant human and animal urokinases involving construction and expression of recombinant expression constructs comprising coding sequences of human or animal urokinases in a plant expression system. The plant expression system provides for post-translational modification and processing to produce a recombinant gene product exhibiting biological activity. The invention is demonstrated by working examples in which transgenic tobacco plants having recombinant expression constructs comprising human urokinase and urokinase nucleotide sequences produced biologically active urokinase. The recombinant urokinases produced in accordance with the invention may be used for a variety of purposes, including but not limited to thrombolytic therapy and clearing catheters.
A method for producing a biologically active urokinase in a transgenic plant, comprising:
A recombinant expression construct comprising a nucleotide sequence encoding a urokinase and a promoter that regulates the expression of the nucleotide sequence in a plant cell.
A transgenic plant or plant cell capable of producing a biologically active urokinase, wherein said plant has a recombinant expression construct comprising a nucleotide sequence encoding a urokinase and a promoter that regulates expression of the nucleotide sequence in the transgenic plant or plant cell.
A urokinase which is biologically active and which is produced according to a process comprising:
Dependencies are inferred from the claim text.