Composition and method for increased meiotic recombination in plants
OwnerAndreas Stefan Betzner, Eric Huttner +3inventors
InventorsAndreas Stefan Betzner, Eric Huttner, Pascual Perez, Marie-Pascale Doutriaux, Charles White
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Abstract
The present invention provides an expression cassette comprising a meiotically active promoter operably linked to a polynucleotide encoding a recombinational DNA repair polypeptide, or fragment thereof, wherein said polynucleotide is capable of stimulating plant meiotic recombination when expressed into RNA and/or said polypeptide.
Claims33
An expression cassette comprising a meiotically active promoter operably linked to a polynucleotide encoding a recombinational DNA repair polypeptide, or fragment thereof, wherein said polynucleotide is capable of stimulating plant meiotic recombination when expressed into RNA and/or said polypeptide.
A method for increasing the frequency of homologous or homeologous recombination in a plant, wherein said method comprises
- a) transforming or transfecting a plant cell or tissue with a polynucleotide encoding a recombinational DNA repair polypeptide, or a fragment thereof, wherein said polynucleotide is capable of stimulating plant meiotic recombination when expressed into RNA and/or said polypeptide, or said polynucleotide is capable of stimulating plant meiotic recombination when introduced into said plant cell or tissue as an RNA::DNA chimeric molecule;
- b) culturing said transformed or transfected plant cell or tissue under conditions allowing the regeneration of a plant,
- c) culturing said regenerated plant under conditions allowing sexual reproduction of said regenerated plant;
- and
- d) expressing said polynucleotide in said regenerated plant;
- e) obtaining a sexually reproduced plant which is the product of said sexual reproduction;
- and
- f) screening said sexually reproduced plant and/or its progeny for homologous or homeologous recombination events.
A method for increasing the frequency of homologous or homeologous meiotic recombination in a plant cell capable of undergoing meiosis, wherein said method comprises transforming or transfecting said plant cell with a polynucleotide encoding a recombinational DNA repair polypeptide, or a fragment thereof wherein said polynucleotide is capable of stimulating meiotic recombination when expressed into RNA and/or said polypeptide, or said polynucleotide is capable of stimulating meiotic recombination when introduced into a plant cell as an RNA::DNA chimeric molecule.
A method for obtaining a plant having a desired characteristic, wherein said method comprises:
- a) transforming or transfecting a plant cell or tissue with a polynucleotide encoding a recombinational DNA repair polypeptide, or a fragment thereof wherein said polynucleotide is capable of stimulating plant meiotic recombination when expressed into RNA and/or said polypeptide, or said polynucleotide is capable of simulating plant meiotic recombination when introduced into said plant cell or tissue as an RNA::DNA chimeric molecule;
- b) culturing said transformed or transfected plant cell or tissue under conditions allowing the regeneration of a plant,
- c) permitting said regenerated plant to self-fertilise to produce a first parent line;
- d) obtaining a hybrid between a plant of the first parent line and a second parent line, or cells thereof;
- e) expressing said polynucleotide in said hybrid plant;
- f) permitting said hybrid plant to self-fertilise and produce offspring plants;
- and
- g) screening said offspring plants for plants having said desired characteristic.
Dependencies are inferred from the claim text.