Method of producing diacylglycerol and gene for inactivating function of gene which encodes diacylglycerol acyltransferase

A soybean diacylglycerol acyltransferase gene used in producing diacylglycerol, characterized by inactivating the function of a gene which encodes diacylglycerol acyltransferase having the function of creating triacylglycerol, said soybean diacylglycerol acyltransferase gene including all or a part of the following sequence.

• 1 61 GTCTTCTTTTCCATGGCGATTTCCGATGAGCCTGAAACTGTAGCCACTGCTCTCAACCAC 120 M A I S D E P E T V A T A L N H 121 TCTTCCCTGCGCCGCCGTCCCACCGCCGCTGGCCTCTTCAATTCGTCCGAGACGACCACC 180 S S L R R R P T A A G L F N S S E T T T 181 GACAGTTCCGGTGATGACTTGGCCAAGGATTCCGGTTCCGACGACTCCATCAGCAGCGAC 240 D S S G D D L A K D S G S D D S I S S D 241 GCCGCCGCCAATTCGCAACCGCAACAAAAACAAGACACTGATTTCTCCGTCCTCAAATTC 300 A A A N S Q P Q Q K Q D T D F S V L K F 301 GCCTACCGTCCTTCCGTCCCCGCTCACCGCAAAGTGAAGGAAAGTCCGCTCAGCTCCGAC 360 A Y R P S V P A H R K V K E S P L S S D 361 ACCATTTTCCGTCAGTTGCAGAGTCACGCGGGCCTCTTCAACCTCTGTATAGTAGTCCTT 420 T I F R Q L Q S H A G L F N L C I Y V L 421 GTTGCTGTGAACAGCAGACTTATCATTGAGAATTTAATGAAGTATGGTTGGTTGATCAAG 480 V A V N S R L I I E N L M K Y G W L I K 481 TATGGCTTTTGGTTTAGTTCAAAATCATTGAGAGATTGGCCTCTCTTCATGTGCTGTCTT 540 Y G F W F S S K S L R D W P L F M C C L 541 AGTCTTGCCATATTTCCACTTGCTGCCTTTGTTGTGGAAAGGTTGGCACAACAAAAGTGT 600 S L A I F P L A A F V V E R L A Q Q K C 601 ATTTCTGAACCAGTTGTTGTTCTACTTCATCTAATAATATCAACTGTTGAACTGTGCTAT 660 I S E P V V V L L H L I I S T V E L C Y 661 CCGGTTTTAGTAATACTCAGGTGTGATTCTGCTTTTGTATCTGGTGTCACGTTGATGCTA 720 P V L V I L R C D S A F V S G V T L M L 721 TTAACTTGCATTGTGTGGTTAKAATTGGTGTCATATGCACATACAAACTATGATATGAGA 780 L T C I V W L K L V S Y A H T N Y D M R 781 GCACTTACTGTTTCGAATGAAAAGGGAGAAACATTACCCAATACTTTGATTATGGAGTAT 840 A L T V S N E K G E T L P N T L I M E Y 841 CCGTACACTGTGACCTTCAGGAGTTTGGCATACTTCATGGTTGCTCCTACATTATGCTAT 900 P Y T V T F R S L A Y F M V A P T L C Y 901 CAGACAAGCTATCCTCGCACACCTTCAGTTCGAAAGGGTTGGGTGTTTCGTCAACTTGTC 960 Q T S Y P R T P S V R K G W V F R Q L V 961 AAGCTGATAATATTTACAGGAGTTATGGGATTTATAATAGAACAATATATGAATCCTATT 1020 K L I I F T G V M G F I I E Q Y M N P I 1021 GTACAAAACTCAACTCATCCTTTGAAGGGKAACCTTCTATATGCCATTGAGAGAATTCTG 1080 V Q N S T H P L K G N L L Y A I E R I L 1081 AAGCTTTCTGTCCCAAATGTATATGTGTGGCTCTGCATGTTCTACTGCTTTTTCCACCTT 1140 K L S V P N V Y V W L C M F Y C F F H L 1141 TGGTTAAATATACTTGCAGAGCTTGTTCGATTTGGTGATCGTGAGTTCTATAAAGATTGG 1200 W L N I L A E L V R F G D R E F Y K D W 1201 TGGAATGCCAAAACTGTTGAAGAGTATTGGAAGATGTGGAATATGCCTGTGCACAAATGG 1260 W N A K T V E E Y W K M W N M P V H K W 1261 ATGGTTCGCCACATATATTTTCCATGCCTAAGGCGTGGTATACCCAAGGGTGCTGCTCCA 1320 M Y R H I Y F P C L R R G I P K G A A P 1321 TTAATTGCATTCCTGGTTTCTGCTGTGTTTCATGAGTTATGCATTGCCGTTCCTTGCCAC 1380 L I A F L V S A V F H E L C I A V P C H 1381 ATGTTCAAGTTGTGGGCTTTTATAGGAATTATGTTTCAGGTTCCTTTGGTCTTGATCACT 1440 M F K L W A F I G I M F Q V P L V L I T 1441 AATTAtCTCCAAAATAAATACAGAAACTCAATGGTTGGAAATATGATTTTTTGGTTCATA 1500 N Y L Q N K Y R N S M V G N M I F W F I 1501 TTTTGTATTCTTGGTCAACCAATGAGCGTACTATTGTACTACCATGACTTGATGAATAGA 1560 F C I L G Q P M S V L L Y Y H D L M N R 1561 AAAGGAGAAGTTGACTAAGGTAGCATTACACTGTTCATGTGGATGAGCTTTTGCGTTTTC 1620 K G E V D *