Sunflower anti-pathogenic proteins and genes and their uses
AssigneesPioneer Hi-Bred International, Inc., CuraGen Corporation
InventorsDennis L. Bidney, Jon Duvick, Xu Hu, Guihua Lu, Oswald R. Crasta
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Abstract
Compositions and methods to aid in protecting plants from invading pathogenic organisms are provided. The compositions of the invention comprise anti-pathogenic genes, including 5′ regulatory sequences in the promoter, and proteins encoded by the anti-pathogenic genes. The compositions find use in methods for reducing or eliminating damage to plants caused by plant pathogens. Transformed plants, plant cells, tissues, and seed having enhanced disease resistance are also provided.
Claims21
A method for increasing resistance of a plant to at least one pathogen, said method comprising transforming said plant with a DNA construct comprising a nucleotide sequence that encodes a protein having anti-pathogenic activity, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3;
- b) the nucleotide sequence set forth in SEQ ID NO:
- 6;
- c) a nucleotide sequence that shares at least 80% identity to the sequence of SEQ ID NO:6;
- and,
- d) the defensin nucleotide sequence contained in a plasmid deposited as Patent Deposit No. PTA-75;
- and,
- e) a nucleotide sequence that hybridizes to the complement of any one of a)-d) under highly stringent conditions, wherein said highly stringent conditions comprise hybridization in 50% formamide, 1M NaCl, 1% sodium dodecyl sulphate at 37° C. for at least 4 hours, and a wash in 0.1×SSC at 60° C. for at least 30 minutes;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell;
- and regenerating stably transformed plants with increased resistance to at least one pathogen.
A plant having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having anti-pathogenic activity, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3;
- b) the nucleotide sequence set forth in SEQ ID NO:
- 6;
- c) a nucleotide sequence that shares at least 80% identity to the sequence of SEQ ID NO:6;
- d) the defensin nucleotide sequence contained in a plasmid deposited as Patent Deposit No. PTA-75;
- and,
- e) a nucleotide sequence that hybridizes to the complement of any one of a)-d) under highly stringent conditions, wherein said highly stringent conditions comprise hybridization in 50% formamide, 1M NaCl, 1% sodium dodecyl sulphate at 37° C. for at least 4 hours, and a wash in 0.1×SSC at 60° C. for at least 30 minutes;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
A plant cell having stably incorporated into its genome a DNA construct comprising a nucleotide sequence that encodes a protein having anti-pathogenic activity, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3;
- b) the nucleotide sequence set forth in SEQ ID NO:6;
- c) a nucleotide sequence that shares at least 80% identity to the sequence of SEQ ID NO:6;
- d) the defensin nucleotide sequence contained in a plasmid deposited as Patent Deposit No. PTA-75;
- and,
- e) a nucleotide sequence that hybridizes to the complement of any one of a)-d) under highly stringent conditions, wherein said highly stringent conditions comprise hybridization in 50% formamide, 1M NaCl, 1% sodium dodecyl sulphate at 37° C. for at least 4 hours, and a wash in 0.1×SSC at 60° C. for at least 30 minutes;
- wherein said nucleotide sequence is operably linked to a promoter that drives expression of a coding sequence in a plant cell.
An isolated nucleic acid molecule having a nucleotide sequence that encodes a protein having anti-pathogenic activity, wherein said nucleotide sequence is selected from the group consisting of:
- a) a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:3;
- b) the nucleotide sequence set forth in SEQ ID NO:6;
- c) a nucleotide sequence that shares at least 80% identity to the sequence of SEQ ID NO:6;
- d) the defensin nucleotide sequence contained in a plasmid deposited as Patent Deposit No. PTA-75;
- and,
- e) a nucleotide sequence that hybridizes to the complement of any one of a)-d) under highly stringent conditions, wherein said highly stringent conditions comprise hybridization in 50% formamide, 1M NaCl, 1% sodium dodecyl sulphate at 37° C. for at least 4 hours, and a wash in 0.1×SSC at 60° C. for at least 30 minutes.
A substantially purified protein molecule having an amino acid sequence selected from the group consisting of:
- a) the amino acid sequence set forth in SEQ ID NO:3;
- b) an amino acid sequence that shares at least 80% sequence similarity to the sequence of SEQ ID NO:3;
- and,
- c) the defensin amino acid sequence encoded by the nucleotide sequence contained in a plasmid deposited as Patent Deposit No. PTA-75.
Dependencies are inferred from the claim text.