Universal carrier for targeting molecules to Gb3 receptor expressing cells
AssigneesInstitute Curie, Centre National De La Recherche Scientifque, Institut National de la Sante et de la Recherche Medicale, Universite Pierre et Marie Curie (Paris VI)
InventorsLudger Johannes, Eric Tartour, Bruno Goud, Wolf Herve Fridman
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Abstract
The present invention concerns an universal polypeptidic carrier for targeting directly or indirectly a molecule to Gb3 receptor expressing cells and having the following formula STxB-Z(n)-Cys, wherein: STxB is the Shiga Toxin B subunit or a functional equivalent thereof, Z is an amino-acid devoided of sulfydryl group, n being 0, 1 or a polypeptide, Cys is the amino-acid Cysteine, and the use thereof for MHC class I and MHC class II presentation of antigens.
Claims24
Universal polypeptidic carrier for targeting directly or indirectly a molecule to Gb3 receptor expressing cells and having the following formula STxB-Z(n)-Cys, wherein:
- STxB is the Shiga Toxin B subunit or a functional equivalent thereof,
- Z is an amino-acid devoided of sulfydryl group, n being 0, 1 or a polypeptide,
- Cys is the amino-acid Cysteine.
An isolated polynucleotide selected from the group consisting of:
- a) a polynucleotide comprising the nucleotide sequence STxB encoding the Shiga Toxin B subunit or a functional equivalent thereof bearing at its 3′end the codon TGT, or the codon TGC encoding Cysteine,
- b) a polynucleotide comprising a nucleotide sequence having at least 80% sequence identity to a nucleotide sequence encoding the Shiga Toxin B subunit or a functional equivalent thereof bearing at its 3′end the codon TGT or TGC,
- c) a nucleotide sequence complementary to the sequence in a) or b).
Method for delivering an sequence of interest into the MHC class I and MHC class II pathway using the a product obtained by covalent binding of the Cys moiety of the universal carrier with said sequence of interest.
Method for delivering an expression vector containing a sequence of interest into a Gb3 receptor expressing cells characterized in that said expression vector is operably linked to a lysinerich peptide covalently linked to the Cys moiety of the universal carrier.
Dependencies are inferred from the claim text.