Mammalian secretory peptide - 9
OwnerPaul O. Sheppard, Laura J. Jelinekinventors
Life of this patent
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Abstract
Novel mammalian secretory polypeptides, polynucleotides encoding the polypeptides (called Zsig9), and related compositions and methods including antibodies and anti-idiotypic antibodies. Overexpression of these proteins is indicative of the presence of cancer. Antibodies and anti-sense nucleotides can be used therapeutically to treat the disease. Furthermore, antibodies to Zsig9 and nucleotide primers and probes can be used to diagnose the presence of tumors.
Claims12
An expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment which encodes a Zsig9 polypeptide or a peptide or polypeptide which contains an epitope-bearing region of a Zsig9 polypeptide;
- and a transcription terminator.
An expression vector comprising the following operably linked elements:
- (a) a transcription promoter;
- (b) a DNA segment encoding a chimeric polypeptide, wherein said chimeric polypeptide consists essentially of a first portion and a second portion joined by a peptide bond, said first portion being comprised of a mammalian polypeptide, said polypeptide being the amino acid sequences of SEQ ID NOs:
- 2-6, 17, 20 19 and 21 and said second portion being a second polypeptide or protein.
- (c) a transcription terminator.
An isolated Zsig9 polypeptide selected from the group of amino acid sequences consisting of SEQ ID NOs:
- 22-6, 17, 20 19 and 21 or a polypeptide which is at least 90% identical to said polypeptides.
An isolated peptide or polypeptide having at least 15 amino acid residues comprised of an epitope-bearing portion of a polypeptide of SEQ ID NOs:
- 2-6, 17, 20 19 and 21.
An antibody, antibody fragment or single-chain antibody that specifically binds to a mammalian polypeptide, said polypeptide being defined by the amino acid sequences of SEQ ID NOs:
- 2-6, 17, 20 19 and 21 or a polypeptide which is at least 90% identical to said amino acid sequences.
A method for producing an antibody which binds to a peptide or polypeptide defined by SEQ ID NOs:
- 2-6, 17, 20 19 and 21 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide comprising bringing into contact a peptide or polypeptide defined by SEQ ID NOs:
- 2-6, 17, 20 19 and 21 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide with a cell capable or producing antibodies or the cell is brought into contact with a nucleic acid which encodes said peptide or polypeptide, wherein said cell produces antibodies to said peptide or polypeptide;
- and isolating said antibody.
An anti-idiotypic antibody, anti-idiotypic antibody fragment or anti-idiotypic single-chain antibody which binds to an antibody, an antibody fragment or single-chain antibody of peptide or polypeptide defined by SEQ ID NOs:
- 2-6, 17, 20 19 and 21 or to a peptide or polypeptide which is at least 90% identical to said peptide or polypeptide.
Dependencies are inferred from the claim text.