Stabilized naked DNA compositions
OwnerRoger C. Adami, Jefferson D. Knight +1inventors
InventorsRoger C. Adami, Jefferson D. Knight, Larry A. Gatlin
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Abstract
The present invention relates to a method to condense DNA without any high-molecular-weight condensing agents by condensing plasmid DNA with a divalent cation and a lyophilizable alcohol. The invention also relates to an aqueous composition which includes condensed plasmid DNA and a carrier such as a lyophilizable, water-miscible alcohol and a divalent cation.
Claims76
An aqueous composition comprising condensed plasmid DNA and a carrier, wherein the carrier comprises a lyophilizable, water-miscible alcohol and a divalent cation.
A method to condense plasmid DNA comprising:
- (a) preparing an aqueous solution of deionized plasmid DNA;
- (b) adding a lyophilizable, water-miscible alcohol to the solution of step (a);
- and
- (c) adding a divalent cation to the mixture of step (b).
A method of protecting DNA against shear stress comprising:
- (a) preparing an aqueous solution of deionized plasmid DNA;
- (b) adding a lyophilizable, water-miscible alcohol to the solution of step (a);
- and
- (c) adding a divalent cation to the mixture of step (b).
A method for preparing a DNA condensate comprising:
- (a) preparing an aqueous solution of deionized plasmid DNA;
- (b) adding a lyophilizable, water-miscible alcohol to the solution of step (a);
- and
- (c) adding a divalent cation to the mixture of step (b),
- wherein said DNA condensate consists essentially of toroids, rods and spheres.
Dependencies are inferred from the claim text.