Methods of reducing ischemic injury
OwnerXinkang Wang, Gary Schieven +1inventors
InventorsXinkang Wang, Gary Schieven, Giora Z. Feuerstein
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Abstract
The present invention includes methods of reducing the activity, such as enzymatic activity and expression, of mitogen-activated protein kinase-activated protein kinase 2. The present invention further includes methods for identifying compounds useful for reducing such activity, and methods for reducing ischemic injury by the administration of such compounds.
Claims26
A method of reducing ischemic injury in a mammal, comprising administering to said mammal a compound that reduces activity of MK2.
A method of reducing ischemic injury in a mammal, comprising administering to said mammal an inhibitor of MK2 expression.
A method of reducing ischemic injury in a mammal, comprising administering to said mammal a compound that reduces activity of MK2.
A method for identifying a compound which inhibits MK2 expression in a cell, said method comprising the steps of:
- (a) providing a cell that expresses MK2;
- (b) culturing said cell in the presence of a test compound;
- and
- (c) determining the level of expression of a MK2 in said cell,
- wherein a decrease in said level of expression in the presence of said test compound compared to the level of expression in the absence of said test compound indicates that said test compound inhibits MK2 expression in said cell.
An assay for identifying a compound that modulates the activity of MK2, comprising:
- (a) providing a cell expressing MK2;
- (b) contacting said cell expressing MK2 with a test compound;
- and
- (c) determining whether said test compound modulates the activity of MK2.
A method for the treatment of ischemic injury, comprising:
- (a) identifying a patient suffering from ischemic injury;
- and
- (b) administering to said patient a therapeutically effective amount of a modulator of MK2.
Dependencies are inferred from the claim text.