Microfluidic affinity system using polydimethylsiloxane and a surface modification process
OwnerCarlo D. Montemagno, Elsa I. Changinventors
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Abstract
A microfluidic affinity system is designed to recognize, capture and separate target analytes from input solutions. This microfluidic affinity system employs fluidic channels fabricated by silicon-based lithography in a silicon substrate. The fluidic channels are patterned and replicated in a substrate, preferably polydimethylsiloxane, PDMS, by pattern transfer from a silicon wafer mold with reversed patterns fabricated by lithography. A novel three-step covalent binding method for surface modification employs the following steps to covalently immobilize an affinity ligand on the substrate: 1) a plasma treatment; 2) a silanization treatment; and 3) a crosslinking treatment.
Claims20
A method for immobilizing an affinity ligand onto a substrate comprising,
- a) subjecting said substrate to a plasma treatment;
- b) subjecting said substrate to a silanization treatment;
- and
- c) subjecting said substrate to a crosslinking treatment.
A microfluidic affinity system comprising,
- a) a substrate subjected to a plasma treatment, a silanization treatment, and a crosslinking treatment;
- and
- b) an affinity ligand bound to said substrate;
Dependencies are inferred from the claim text.