Nucleic acid constructs and methods for producing altered seed oil compositions
OwnerJoAnne J. Fillatti, Neal A. Bringe +1inventors
InventorsJoAnne J. Fillatti, Neal A. Bringe, Katayoon Dehesh
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Abstract
The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions.
Claims74
A soybean seed exhibiting an oil composition comprising 55 to 80% by weight oleic acid, 10 to 40% by weight linoleic acid, 6% or less by weight linolenic acid, and 2 to 8% by weight saturated fatty acids.
A container of soybean seeds, wherein at least 25% of the seeds exhibit an oil composition comprising 55 to 80% by weight oleic acid, 10 to 40% by weight linoleic acid, 6% or less by weight linolenic acid, and 2 to 8% by weight saturated fatty acids.
A soybean seed exhibiting an oil composition comprising 65 to 80% by weight oleic acid, 10 to 30% by weight linoleic acid, 6% or less by weight linolenic acid, and 2 to 8% by weight saturated fatty acids.
A crude soybean oil exhibiting an oil composition comprising 55 to 80% by weight oleic acid, 10 to 40% by weight linoleic acid, 6% or less by weight linolenic acid, and 2 to 8% by weight saturated fatty acids.
A crude soybean oil exhibiting an oil composition comprising 65 to 80% by weight oleic acid, 10 to 40% by weight linoleic acid, 6% or less by weight linolenic acid, and 2 to 8% by weight, saturated fatty acids.
A crude soybean oil exhibiting an oil composition which comprises 69 to 73% by weight oleic acid, 21 to 24% by weight linoleic acid, 0.5 to 3% by weight linolenic acid, and 2-3% by weight of saturated fatty acids.
A transformed soybean plant bearing seed, wherein said seed exhibits an oil composition which comprises 55 to 80% by weight oleic acid, 10 to 40% by weight linoleic acid, 6% or less by weight linolenic acid, and 2 to 8% by weight saturated fatty acids.
A transformed plant comprising a recombinant nucleic acid molecule which comprises
- a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of at least two genes selected from the group consisting of FAD2, FAD3, and FATB genes, and
- a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, and a delta-9 desaturase gene.
A method of altering the oil composition of a plant cell comprising:
- (A) transforming a plant cell with a recombinant nucleic acid molecule which comprises a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of at least two genes selected from the group consisting of FAD2, FAD3, and FATB genes, and a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, and a delta-9 desaturase gene;
- and
- (B) growing said plant cell under conditions wherein transcription of said first set of DNA sequences and said second set of DNA sequences is initiated, whereby said oil composition is altered relative to a plant cell with a similar genetic background but lacking the recombinant nucleic acid molecule.
A method of producing a transformed plant having seed with a reduced saturated fatty acid content comprising:
- (A) transforming a plant cell with a recombinant nucleic acid molecule which comprises a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of at least two genes selected from the group consisting of FAD2, FAD3, and FATB genes, and a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, and a delta-9 desaturase gene;
- and
- (B) growing the transformed plant, wherein the transformed plant produces seed with a reduced saturated fatty acid content relative to seed from a plant having a similar genetic background but lacking the recombinant nucleic acid molecule.
A recombinant nucleic acid molecule comprising:
- a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of at least two genes selected from the group consisting of FAD2, FAD3, and FATB genes;
- and
- a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, and a delta-9 desaturase gene.
A recombinant nucleic acid molecule comprising:
- a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of a FAD2 gene and a FAD3 gene, wherein said first set of DNA sequences comprises a first non-coding sequence that expresses a first RNA sequence that exhibits at least 90% identity to a non-coding region of a FAD2 gene, a first antisense sequence that expresses a first antisense RNA sequence capable of forming a double-stranded RNA molecule with the first RNA sequence, a second non-coding sequence that expresses a second RNA sequence that exhibits at least 90% identity to a non-coding region of a FAD3 gene, and a second antisense sequence that expresses a second antisense RNA sequence capable of forming a double-stranded RNA molecule with the second RNA sequence;
- and a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, and a delta-9 desaturase gene.
A recombinant nucleic acid molecule comprising:
- a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of a FAD2 gene and a FAD3 gene;
- and
- a second set of DNA sequences that comprises a first coding sequence that is capable of expressing a CP4 EPSPS gene, and a second coding sequence that is capable, when expressed, of increasing the endogenous expression of a gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, and a delta-9 desaturase gene.
A nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 29, 30, and 31.
A nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOS:
- 44, 45, 46, and 47.
Dependencies are inferred from the claim text.