Nuclear transfer with embryonic stem cells

A method for producing a non-human animal with a nuclear genome of interest, said method comprising the steps of:

• (a) providing an embryonic stem cell comprising a nucleus having a genome of interest of a non-human animal;
• (b) culturing an oocyte of the nonhuman animal so that it is a matured and activated oocyte or production of a zygote in vivo or in vitro;
• (c) enucleating the matured oocyte or zygote of step (b) to produce an enucleated recipient cell;
• (d) transferring the nucleus of step (a) to the enucleated recipient cell of step (c) to produce a nuclear transfer embryo;
• (e) culturing a nuclear transfer embryo of step (d) to produce an embryo of a 4-cell, 8-cell, 16-cell, compact morula or blastocyst stage of development;