Method and device for determining state of skin surface

A method or measuring the degree of skin stimulation, which comprises:

• floating a skin section, with its surface upward, on the surface of a culture solution accommodated in a container;
• measuring a potential difference by imparting stimulation to the skin surface upon measuring of a potential difference between the skin surface of the skin section and the culture solution;
• and determining the degree of skin stimulation from a difference between the measured potential difference value and a potential difference when no stimulation is imparted.

A method for measuring the degree of skin stimulation, which comprises:

• floating a skin section, with its surface upward, on the surface of a culture solution accommodated in a container;
• measuring a potential difference by adding a stimulating substance to a culture solution upon measuring a potential difference between the skin surface of the skin section and said culture solution;
• and determining the degree of skin stimulation from a difference between the measured potential difference value and a potential difference when no stimulating substance is added.

A method for measuring the degree of inhibition of skin stimulation by a drug, which comprises:

• floating a stimulated skin section, with its surface upward, on the surface of a culture solution accommodated in a container;
• measuring a potential difference by coating a drug on the skin surface, either before or after imparting stimulation to the skin surface upon measuring a potential difference between the skin surface of the skin section and said culture solution;
• and determining the degree of inhibition of skin stimulation by the drug from a difference between the measured potential difference value and a potential difference when no drug is coated.

A method for measuring the degree of inhibition of skin stimulation by a drug, which comprises floating a stimulated skin section, with its surface upward, on the surface of a culture solution accommodated in a container;

• measuring a potential difference by adding a drug to the culture solution, either before or after imparting stimulation to the skin surface upon measuring a potential difference between the skin surface of the skin section and said culture solution;
• and determining the degree of inhibition of skin stimulation by the drug from a difference between the measured potential difference value and a potential difference when no drug is added.

A method for measuring the degree of inhibition of skin stimulation by a drug, which comprises:

• floating a skin section, with its surface upward, on the surface of a culture solution accommodated in a container, with a stimulating substance added thereto;
• measuring a potential difference by coating a drug on the skin surface either before or after adding the stimulating substance to the culture solution upon measuring a potential difference between the skin surface of the skin section and said culture solution;
• and determining the degree of inhibition of skin stimulation by the drug from a difference between the measured potential difference value and a potential difference when no drug is coated.

A method for measuring the degree of skin stimulation by a drug which comprises:

• floating a skin section, with its surface upward, on the surface of a culture solution accommodated in a container, with a stimulating substance added thereto;
• measuring a potential difference by adding a drug to the culture solution either before or after adding the stimulating substance to the culture solution upon measuring a potential difference between the skin surface of the skin section and dead culture solution;
• and determining the degree of inhibition of skin stimulation by the drug from a difference between the measured potential difference value and a potential difference when no stimulating substance is added.

A method for measuring the skin surface condition, which comprises using a sublingual potential as a standard potential upon measuring the skin surface condition.

A method for measuring the skin surface condition, which comprises measuring the skin surface condition from a potential difference between a standard electrode part communicating with a sublingual area and a measuring electrode part communicating to the skin surface.

The skin surface condition measuring apparatus, which comprises a standard electrode part communicating to a sublingual area, a measuring electrode part communicating to the skin surface, and a potential difference detecting part for detecting a potential difference between said standard electrode part and said measuring electrode part.