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US 2004/0106567 A1Published application
Intravascular delivery of non-viral nucleic acid
OwnerJames E. Hagstrom, Jon A. Wolff +5inventors
InventorsJames E. Hagstrom, Jon A. Wolff, Sean D. Monahan, David B. Rozema, Vladimir G. Budker, Paul M. Slattum, David L. Lewis
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Abstract
The process comprises designing a polynucleotide, such as an siRNA, for transfection. The polynucleotide is inserted into a mammalian vessel such as an artery. Prior to insertion, subsequent to insertion, or concurrent with insertion volume in the vessel is increased allowing the polynucleotide delivery to the parenchymal cell.
Claims8
A process for inhibiting expression of a gene in an extravascular mammalian cell comprising:
- a) injecting a naked polynucleotide into a blood vessel lumen, in vivo;
- b) increasing permeability in the blood vessel;
- and, c) delivering the naked polynucleotide to an extravascular cell outside of the blood vessel via the increased permeability.
Dependencies are inferred from the claim text.