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US 2004/0106565 A1Published application
Gene expression, genome alteration and reporter expression in myofibroblasts and myofibroblast-like cells
OwnerMargarete Odenthal, Diana Junginventors
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Abstract
The invention relates to specific, regulatory sequence regions of the alpha smooth muscle actin gene (a-SMA gene), and fusion structures in which said regulatory sequence regions of the a-SMA gene are operationally linked to other functional nucleic acid sequences. The invention also relates to the use of said regulatory sequence regions for cell type specific and differentiation specific reporter expression, for cell type specific and differentiation specific gene changes (alteration, mutation) and for gene expression changes in cells and organisms.
Claims26
Use of a nucleic acid sequence comprising
- (i) one or more functional regions of the regulatory sequence region of the alpha-smooth muscle actin gene (α-SMA gene) that consists of nucleotides −698 to +18 of the α-SMA gene, wherein the numbering refers to the sequence of rattus norvegicus as shown in FIGS. 5A and 7A, and
- (ii) at least one additional functional nucleic acid sequence that does not encode for the natural α-SMA and that is operatively linked with sequence (i)
- for the preparation of a pharmaceutical composition for manipulation of gene expression and/or cell function of transient or after activation, SMA-positive cells, of myofibroblasts or myofibroblast-like cells.
A nucleic acid sequence, comprising
- (i) one or more functional regions of the regulatory sequence region of the alpha-smooth muscle actin gene (α-SMA gene) that consists of nucleotides −698 to +18 of the α-SMA gene, wherein the numbering refers to the sequence from rattus norvegicus as shown in FIGS. 5A and 7A, and
- (ii) at least one additional functional nucleic acid sequence that is operatively linked with sequence (i) and does not encode a natural α-SMA and is selected from DNA sequences that encode pharmacologically active proteins, regulatory sequences or functional RNA encoding sequences.
Dependencies are inferred from the claim text.