Method of measuring drug-metabolizing enzyme activity, method of evaluating inhibition of drug-metabolizing enzyme activity, and composition for these methods

A method of evaluating the degree of effect of a third substance or substances on the effect that other substance or substances may have on the intended action of a certain substance or substances, comprising the steps of:

• applying said other substance or substances to said certain substance or substances in the presence of the third substance or substances;
• and
• measuring changes in the amount of the resulting product by an immunochemical method to determine the intended action of said certain substance or substances.

A method of measuring a drug-metabolizing enzyme activity the method comprising the steps of:

• applying the drug-metabolizing enzyme to a substrate or substrates (particularly an endogenous unmodified substrate or substrates);
• and
• assaying the resulting product by an immunochemical method, preferably within 3 hours.

A method of measuring drug-metabolizing enzyme activity comprising the steps of:

• applying the drug-metabolizing enzyme to a substrate or substrates (particularly an endogenous unmodified substrate or substrates) in the presence of a chemical substance or substrates to be evaluated;
• and
• determining changes in the amount of the resulting product by an immunochemical method, with preferably at least 10 samples, more preferably at least 28 samples, or further more preferably at least 96 samples measured within 3 hours, and the effect of the chemical substance of interest is evaluated, to evaluate the effect of the chemical substance to be evaluated.

A reagent composition for measuring the degree to which a certain substance or substances are affected by other substance or substances which may have an effect on an intended action of said certain substance or substances, the reagent composition comprising (1)-(3):

• (1) other substance or substances which may have an effect on the intended action of said certain substance or substances,
• (2) at least one antibody which specifically recognizes the product that results when a certain substance or substances are affected by other substance or substances which may have an effect on an intended action of said certain substance or substances,
• (3) at least one reagent which allows measurement using the antibody described in (2) according to one of the methods consisting of enzyme immunoassay, surface plasmon resonance, micro-differential thermal measurement or quartz resonance.

A kit for measuring the degree to which a certain substance or substances are affected by other substance or substances which may have an effect on an intended action of said certain substance or substances, the component parts of which are selected as needed from among at least one plate immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products) or at least one gold thin-film chip immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products), at least one quartz resonator chip immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products), at least one reaction buffer, at least one specific antibody solution, at least one antibody sample diluent, at least one standard substance, at least one tracer enzyme substrate liquid for assaying the tracer enzyme, and the like.

A method of evaluating differences between individuals or between groups of humans in metabolizing enzyme activity with respect to a drug, comprising the steps of:

• applying a drug-metabolizing enzyme to the drug;
• and
• assaying the resulting product by an immunochemical method to determine an activity of the drug-metabolizing enzyme.

A method of evaluating differences between individuals or between groups of humans in inhibition of drug-metabolizing enzyme activity with respect to a drug, comprising the steps of:

• applying the drug-metabolizing enzyme to a substrate or substrates in the presence of a chemical substance or substances to be evaluated;
• and
• measuring changes in the amount of the resulting product by an immunochemical method to assay the activity of the drug-metabolizing enzyme.

A kit for evaluating differences between individuals or between specific groups of humans in metabolizing enzyme activity with respect to a drug, the component parts of which are selected as needed from among at least one plate immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products) or at least one gold thin-film chip immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products), at least one quartz resonator chip immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products), at least one reaction buffer, at least one specific antibody solution, at least one antibody sample diluent, at least one standard substance, at least one tracer enzyme substrate liquid for assaying the tracer enzyme, and the like.

A kit for evaluating differences between individuals or between specific groups of humans in inhibition of metabolizing enzyme activity with respect to a drug, the component parts of which are selected as needed from among at least one plate immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products) or at least one gold thin-film chip immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products), at least one quartz resonator chip immobilizing a specific antibody or antibodies (a protein or proteins to cross-link a product or products), at least one reaction buffer, at least one specific antibody solution, at least one antibody sample diluent, at least one standard substance, at least one tracer enzyme substrate liquid for assaying the tracer enzyme, and the like.