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US 2004/0106206 A1Published application
Laser capture microdissection vacuum hold-down
AssigneeArcturus Bioscience, Inc.
InventorsThomas M. Baer, Mark A. Enright, David F. Head, Christopher E. Todd
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Abstract
Systems and methods for laser capture microdissection are disclosed. An inverted microscope includes a vacuum chuck subsystem that is adapted to hold a microscope slide down during the process of laser capture microdissection. The systems and methods provide the advantages of increased speed and much lower rates of contamination.
Claims28
A laser capture microdissection method, comprising:
- providing a sample that is to undergo laser capture microdissection;
- positioning said sample on a translation stage of a laser capture microdissection instrument and within an optical axis of said laser capture microdissection instrument, said translation stage including a vacuum chuck having a beam path hole through which said optical axis extends;
- holding a sample holder in a position over said beam path hole, said sample being located upon said sample holder;
- providing a transfer film carrier having a substrate surface and a laser capture microdissection transfer film coupled to said substrate surface;
- placing said laser capture microdissection transfer film in juxtaposition with said sample with a pressure sufficient to allow laser capture microdissection transfer of a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdisection film;
- and then
- transferring a portion of said sample to said laser capture microdissection transfer film, without forcing nonspecific transfer of a remainder of said sample to said laser capture microdissection transfer film.
4 more elements
A laser capture microdissection instrument, comprising:
- a translation stage;
- and
- a vacuum chuck coupled to said translation stage.
An inverted microscope, comprising:
- a translation stage;
- and
- a vacuum chuck connected to said translation stage.
Dependencies are inferred from the claim text.