Charged cyclodextrin derivatives and their use in plant cell and tissue culture growth media
OwnerAlexey V. Eliseevinventor
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Abstract
The invention provides cyclodextrin derivatives that are substituted with groups bearing charges in aqueous solutions (charged cyclodextrins) in their salt forms and their use, also in combinations with other cyclodextrins, as useful components of plant cell and tissue growth media. The invention also comprises a new method of isolation of useful hydrophobic compounds, such as taxol, produced by plant cultures from the cyclodextrin-containing growth media and from the corresponding cell cultures.
Claims21
A composition of matter, comprising a monopotassium salt of cyclodextrin-6A-monophosphate.
A composition of matter, comprising a monopotassium salt of cyclodextrin-6A-monosuccinylate.
A growth medium for plant cell or tissue cultures, comprising at least one charged cyclodextrin.
A method of growing plant cell or tissue cultures, comprising contacting plant cell or tissue cultures from the genus taxus with a growth medium comprising at least one charged cyclodextrin.
A method of isolating hydrophobic compounds produced by plant cell or tissue cultures, the plant cell or tissue cultures growing in cyclodextrin containing media, the method comprising separating at least one cylcodextrin complex with at least one hydrophobic compound by size exclusion chromatography, followed by dissociating of the at least one cyclodextrin complex.
A composition of matter, comprising a salt of a cationic cyclodextrin of a plant nutrient.
A composition of matter, comprising a dipotassium salt of cyclodextrin-6-bisphosphate.
Dependencies are inferred from the claim text.