Proteins in a porous support
AssigneeBattelle Memorial Institute
InventorsEric Ackerman, Jun Liu
Life of this patent
Estimating the term…
Abstract
A protein system is described in which a protein is bound within a matrix material that has pores that are sized to achieve excellent properties such as: activity, protein density, and stability. In a preferred embodiment, the pore sizes range from 50 to 400 Å. One protein that has demonstrated surprisingly good results in this system is OPH. This protein is known to degrade organophosphorus compounds such as are found in chemical weapons and pesticides. Novel methods of forming the protein system and methods of making OPH are also described.
Claims39
A protein system comprising:
- a porous matrix material having a pore volume wherein at least 90% of the pore volume is composed of pores having sizes in the range of 50 to 400 Å, and
- further comprising a chemically-active protein bonded to the matrix material.
A protein system comprising:
- a porous matrix material being sized such that the protein system comprises 0.01 to 1 mmol of protein per gram of matrix material and wherein said protein in the protein system exhibits an activity of at least 65% that of the activity of the protein in the active state.
A method of forming a protein system comprising the steps of:
- providing a porous matrix material having a pore volume wherein at least 90% of the pore volume is composed of pores having sizes in the range of 50 to 400 Å, and
- reacting the porous matrix material with a protein so that said protein chemically bonds to the porous matrix material.
A method for producing OPH comprising:
- transfecting a host cell with said vector comprising a sequence encoding OPH, said sequence being operably linked to a T7 expression control sequence, culturing said transfected host cell under conditions permitting expression under the control of said expression control sequence, and purifying said OPH from the cell or the medium of the cell.
Dependencies are inferred from the claim text.