Peptides with anti-hypertensive properties
AssigneeChr. Hansen A/S
InventorBenedicte Flambard
Life of this patent
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Abstract
Use of a lactic acid bacterium comprising a cell wall proteinase of around 200 kDa to prepare peptides with anti-hypertensive properties and a method for obtaining such a lactic acid bacterium.
Claims13
A process for preparing peptides with anti-hypertensive properties, the process comprising fermenting a food material, comprising animal milk proteins or vegetable proteins, with a lactic acid bacterium to obtain a fermented food material which comprises the peptides with anti-hypertensive properties, characterized in that the lactic acid bacterium comprises a gene sequence encoding a cell wall proteinase (termed prtH200), wherein the gene sequence is defined as
- prtH200 is a DNA sequence encoding an enzyme exhibiting cell wall proteinase activity, which DNA sequence is selected from the group consisting of:
- (a) the DNA sequence shown in positions 1-5550 in SEQ ID NO 1;
- (b) a DNA sequence comprising a fragment of at least 75 base pairs (bp) that is at least 50% identical to a corresponding fragment of the DNA sequence defined in (a);
- (c) a DNA sequence that encodes a polypeptide, exhibiting cell wall proteinase activity, comprising a fragment of at least 200 amino acids (aa) that is at least 30% identical to a corresponding fragment of the polypeptide sequence shown in positions 1-1849 of SEQ ID NO 2;
- (d) a DNA sequence which hybridizes with a double-stranded DNA probe comprising the DNA sequence shown in positions 1-5550 in SEQ ID NO 1 at low stringency;
- and
- (e) a DNA sequence which is a fragment of the DNA sequences specified in (a), (b), (c), or (d).
The process for preparing peptides of any of the preceding claims, wherein the food material comprises animal milk proteins.
The process for preparing peptides of any of the preceding claims, wherein the lactic acid bacterium is a bacterium of the phylium Firmicutes, more preferably of the class Bacilli, even more preferably of the order Lactobacillales.
A Lactobacillus helveticus bacterium with the registration number DSM 14998 or a mutant thereof.
Dependencies are inferred from the claim text.