Enzyme electrode and process for manufacturing the same

An enzyme electrode comprising:

• a portion of electrode formed on an insulating substrate;
• an electrode protective layer covering the portion of electrode;
• a binding layer comprising a silane-containing compound, which is formed on the electrode protective layer;
• an ion-exchange resin film layer formed on the binding layer;
• an immobilized enzyme layer formed on the ion-exchange resin film layer;
• an adhesion layer comprising a silane-containing compound, which is formed on the immobilized enzyme layer;
• and
• a permeation-limiting layer comprising a fluorine-containing polymer having a structure where a pendant group containing at least a fluoroalkylene block is attached to an unfluorinated vinyl-based polymer, which is formed on the adhesion layer.

An enzyme electrode comprising:

• a portion of electrode formed on an insulating substrate;
• an immobilized enzyme layer formed on the portion of electrode;
• and
• a permeation-limiting layer formed over the immobilized enzyme layer and placed on the uppermost surface;
• wherein said permeation-limiting layer consists of a film essentially comprising a fluorine-containing polymer, and many grooves are built in on the surface of said permeation-limiting layer consisting of the film essentially comprising a fluorine-containing polymer.

A process for manufacturing an enzyme electrode comprising the steps of:

• forming an electrode film on the main surface of an insulating substrate and then patterning the electrode film to form a plurality of portions of electrodes;
• forming an electrode protective layer covering the electrode surface;
• forming a binding layer comprising a silane-containing compound on the main surface of the insulating substrate;
• forming an ion-exchange resin film layer on the main surface of the insulating substrate;
• applying an liquid containing an enzyme to the main surface of the insulating substrate and then drying the insulating substrate to form an immobilized enzyme layer;
• applying a liquid containing a fluorine-containing polymer having a structure where a pendant group comprising at least a fluoroalkylene block is attached to an unfluorinated vinyl-based polymer to the main surface of the insulating substrate by spin coating and then drying the insulating substrate to form the permeation-limiting layer;
• and
• dicing the insulating substrate to give a plurality of enzyme electrodes.