Screening assay for antagonists of human leukocyte receptors
OwnerZhenyi Xu, K M Erik Michaelsson +2inventors
InventorsZhenyi Xu, K M Erik Michaelsson, Leif Petersson, Paul Sorensen
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Abstract
A method of screening compounds for their ability of inhibiting ligand-induced co-stimulatory receptor internalisation pathways in immune competent human cells is described. The immune competent human cells are incubated at conditions capable of inducing co-stimulatory receptor internalisation in the presence of at least one test compound and the suppression of the ligand-induced co-stimulatory receptor internalisation is determined. There is also described a kit for use in such a method, as well as an immunoregulatory drug capable of blocking down-modulation of a ligand-induced receptor.
Claims20
A method of screening compounds for their ability of inhibiting ligand-induced co-stimulatory receptor internalisation pathways in immune competent human cells, characterised by
- incubating said immune competent cells at conditions capable of inducing co-stimulatory receptor internalisation in the presence of at least one test compound;
- and
- determining the suppression of the ligand-induced co-stimulatory receptor internalisation.
A kit for use in screening compounds for their ability of inhibiting ligand-induced co-stimulatory receptor internalisation in immune competent human cells, comprising
- means for culturing immune competent human cells;
- means for inducing co-stimulatory-receptor internalisation;
- means for incubating the immune competent human cells with at least one test compound;
- means for marking the receptors;
- and
- means for determining suppression of the ligand-induced co-stimulatory receptor internalisation.
An immuno-regulatory drug, capable of blocking down-modulation of a ligand-induced receptor thus preventing ligand-induced receptor internalisation.
Dependencies are inferred from the claim text.