Novel zwitterionic fluorescent dyes for labeling in proteomic and other biological analyses
AssigneeMontana State University
InventorsEdward A. Dratz, Paul A. Grieco
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Abstract
The invention relates to compositions and methods useful in the labeling and identification of proteins. The invention provides for highly soluble zwitterionic dye molecules where the dyes and associated side groups are non-titratable and maintain their net zwitterionic character over a broad pH range, for example, between pH 3 and 12. These dye molecules find utility in a variety of applications, including use in the field of proteomics.
Claims27
An optical labeling molecule comprising:
- a. a zwitterionic dye moiety;
- b. a titrable group moiety;
- and
- c. a functional linker moiety.
A method of labeling a target protein comprising the steps of:
- a. providing an optical labeling molecule comprising
- i. a zwitterionic dye moiety;
- ii. a titratable group moiety;
- iii. an optional cleavable moiety;
- and
- iv. a functional linker moiety;
- b. contacting the target protein with the labeling molecule to form a labeled protein.
A method of performing protein analysis on a plurality of proteins comprising:
- a. providing a plurality of different labeled proteins, each comprising a
- i. a different zwitterionic dye moiety;
- ii. a titratable group moiety;
- and
- iii. an optional cleavable moiety;
- b. determining the presence or absence of each of the different labeled proteins.
Dependencies are inferred from the claim text.