In vivo screening array
AssigneeThe General Hospital Corporation
InventorG. Paolo Dotto
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Abstract
The invention features methods of evaluating the effect of a plurality of different treatments or compounds on a tissue. The method includes providing a tissue from an animal; contacting the tissue with a plurality of treatment, forming on the tissue an array of a plurality of regions or addresses. The method includes the use of a two dimensional array member to contact the plurality of treatments with the tissue and/or to evaluate their effect. The methods are useful for screening large numbers of therapeutic or cosmetic compounds.
Claims14
A method of evaluating the effect of a plurality of treatments on a tissue of a subject, the method comprising:
- providing a tissue from an animal;
- contacting the tissue with the plurality of treatments to thereby form on the tissue an array of a plurality of individually addressable regions or addresses, each region or address of the plurality being provided with test treatment which differs from those at other addresses of the plurality;
- and
- evaluating a parameter at each of the plurality of regions or addresses;
- and where either or both the contacting of the compounds with the tissue to form the array, or the evaluation of the parameter, is performed with a two dimensional array member which is contacted with the tissue,
- thereby evaluating the effect of a plurality of test compounds.
A method of evaluating the effect of a plurality of test compounds on a tissue of a subject, the method comprising:
- providing a transgenic animal having a transgene comprising a nucleic acid which encodes a reporter molecule functionally linked to the control region of a first gene;
- contacting a tissue from the transgenic animal with the plurality of test compounds to thereby form on the tissue an array of a plurality of individually addressable regions or addresses, each region or address of the plurality including a test compound which differs from those at other addresses of the plurality;
- and
- evaluating a signal produced by the reporter molecule at each of the plurality of regions or addresses, the presence or strength of which is correlated with the expression of the first gene;
- and where either or both the contacting of the compounds with the tissue to form the array, or the evaluation of signal is performed, with a two dimensional array member which is contacted with the tissue,
- thereby evaluating the effect of a plurality of test compounds.
A method of evaluating the effect of a plurality of different treatments on a tissue, the method comprising:
- providing a tissue having a reporter agent which produces a signal which is correlated with a biological state;
- contacting the tissue with a plurality of different treatments, or a plurality of different concentrations or dosages of a treatment, to thereby form on the tissue, an array of a plurality of individually addressable regions or addresses, each region or address of the plurality including a test treatment which differs from those at other addresses of the plurality;
- and
- evaluating a signal produced by the reporter agent at each of the plurality of regions or addresses,
- where, optionally, either or both the contacting of the compounds with the tissue to form the array, or the evaluation of signal is performed, with a two dimensional array member which is contacted with the tissue,
- thereby evaluating the effect of a plurality of different treatments.
Dependencies are inferred from the claim text.