Method for monitoring the temperature of a biochemical reaction
AssigneeThe Secretary of State for Defence
InventorsMartin A. Lee, Gale Brightwell
Life of this patent
Estimating the term…
Abstract
A method of monitoring the temperature of a biochemical reaction such as an amplification reaction is described. The method comprises effecting the reaction in the presence of a fluorescently labeled temperature probe DNA sequence which comprises a double stranded region which denatures at a predetermined temperature, the fluorescent label of said temperature probe sequence being arranged so that a detectable signal occurs at the point at which denaturation of said region takes places; and monitoring fluorescence from said reaction mixture so as to determine when said predetermined temperature has been reached.
Claims16
A method of monitoring the temperature of a biochemical reaction, said method comprising effecting the reaction in the presence of a fluorescently labelled temperature probe DNA sequence which comprises a double stranded region which denatures at a predetermined temperature, the fluorescent label of said temperature probe sequence being arranged so that a detectable signal occurs at the point at which denaturation of the said region takes place;
- and monitoring fluorescence from said reaction mixture so as to determine when the said predetermined temperature has been reached.
A method according to any one of the preceding claims wherein the fluorescent label comprises an intercalating dye.
A method according to any one of the preceding claims wherein the length of the temperature probe sequence is used to set the said predetermined temperature.
A method according to any one of the preceding claims wherein the GC content of the temperature probe sequence is modified to obtain the desired predetermined temperature.
A method according to any one of the preceding claims wherein the biochemical reaction is an amplification reaction.
Dependencies are inferred from the claim text.