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OwnerMichael E. O'Donnell, Alexander Yuzhakov +4inventors
InventorsMichael E. O'Donnell, Alexander Yuzhakov, Olga Yurieva, David Jeruzalmi, Irina Bruck, John Kuriyan
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The present invention relates to an isolated DNA molecule from a thermophilic bacterium which encodes a DNA polymerase III-type enzyme subunit. Also encompassed by the present invention are host cells and expression system including the heterologous DNA molecule of the present invention, as well as isolated replication enzyme subunits encoded by such DNA molecules. Also disclosed is a method of producing a recombinant thermostable DNA polyrnerase III-type enzyme, or subunit thereof, from a thermophilic bacterium, which is carried out by transforming a host cell with at least one heterologous DNA molecule of the present invention under conditions suitable for expression of the DNA polymerase III-type enzyme, or subunit thereof, and then isolating the DNA polymerase III-type enzyme, or subunit thereof.
An isolated DNA molecule from a Bacillus species encoding a single-strand binding protein, the isolated DNA molecule either:
An isolated DNA molecule from Bacillus stearothermophilus encoding a single-strand binding protein, wherein the single-strand binding protein can bind to ssDNA to remove secondary structure elements from the ssDNA.
Dependencies are inferred from the claim text.