Sensor arrangement, device and method for testing active substances and/or active sites from a pharmacological point of view using an amperometer and/or potentiometer
OwnerKlaus Fendlerinventor
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Abstract
The invention relates to a sensor electrode device (20) for carrying out amperometric and/or potentiometric testing of active sites or substances from a pharmacological point of view. In order to carry out said testing in a reliable, quick and inexpensive manner, said device is provided with a solid-supported electrode area (21) and is electrically insulated in relation to the measuring medium (30) and primary supports (10) mounted on the electrode area. Vesicules and membrane fragments, inter alia, are used as primary supports.
Claims17
Sensor arrangement for pharmacological testing of an active site and/or active ingredient using amperometric and/or potentiometric means:
- with a secondary carrier (20) which has an electrically conductive and solid-like electrode area (21),
- with a plurality of primary carriers (10) which are located in the immediate spatial vicinity of the secondary carrier (20) and which have biological units (12), especially membrane proteins, which can be activated into electrical action,
- with an aqueous measurement medium (30) which contains the primary carriers (10) and the secondary carriers (20),
- the electrode area (21) being made electrically insulated relative to the measurement medium (30),
- the electrode area (21) being made electrically insulated relative to the primary carriers (10) and relative to the biological units (12),
- and the primary carrier (10) can be a eukaryotic cell, a procaryotic cell, a bacterium, a virus, or components, especially membrane fragments, or associations thereof in native form or in altered form, especially in purified, microbiologically and/or molecular biologically altered form, or,
- the primary carriers (10) can be a vesicle, a liposome or a micellar structure.
Sensor arrangement as claimed in one of the preceding claims, wherein the biological unit (12) is made to be activated to electrogenic charge carrier movement, especially to electrogenic charge carrier transport.
Sensor arrangement as claimed in one of the preceding claims, wherein the biological unit (12) can be a membrane protein, especially an ion pump, an ion channel, a transporter, a receptor or a component or an association thereof.
Sensor arrangement as claimed in one of the preceding claims, wherein the biological unit (12) is provided in native form or in altered form, especially in purified, microbiologically and/or molecular biologically altered form.
Sensor arrangement as claimed in one of the preceding claims, wherein
- the surface (10a) of the primary carrier (10) and the surface of the secondary carrier (20) are made polarly opposite or oppositely charged to one another and/or
- wherein between the surface (10a) of the primary carrier (10) and the surface of the secondary carrier (20) coupling in the manner of a chemical bond is formed, especially via his-tag coupling or streptavidin-biotin coupling.
Dependencies are inferred from the claim text.