Simplified detection process for colored bead random microarrays
AssigneeEastman Kodak Company
InventorThomas E. Kocher
Life of this patent
Estimating the term…
Abstract
A method of manufacturing a colored microsphere molecular biological/chemical random microarray system, comprising: randomly dispersing on a support a composition including a first set of microspheres modified with a biological/chemical probe and containing an optical bar code generated from at least one colorant associated with the microspheres to produce a biologically/chemically active region on the support; scanning the biologically/chemically active region with a high-resolution color scanner to produce a color map of the locations of the randomly dispersed first set of microspheres; creating a digital file of the color map; and linking the digital file of the color map with the support.
Claims15
A method of manufacturing a colored microsphere molecular biological/chemical random microarray system, comprising:
- randomly dispersing on a support a composition including a first set of microspheres modified with a biological/chemical probe and containing an optical bar code generated from at least one colorant associated with said microspheres to produce a biologically/chemically active region on said support;
- scanning said biologically/chemically active region with a high-resolution color scanner to produce a color map of the locations of said randomly dispersed first set of microspheres;
- creating a digital file of said color map;
- and
- linking said digital file of said color map with said support.
A colored microsphere molecular biological/chemical random microarray system comprising:
- a support;
- a biologically/chemically active region on said support, said region having a randomly dispersed composition (a) including a first set of microspheres modified with a biological/chemical probe and (b) containing an optical bar code generated from at least one colorant associated with the microspheres;
- and
- a data region linked to said support outside of said active region having a stored digital file of a color map of the locations of said randomly dispersed first set of microspheres.
A method of identifying at least one biological/chemical analyte in a sample containing said analyte comprising:
- providing a colored microsphere molecular biological/chemical random microarray system including (I) a support having a biologically/chemically active region with a randomly dispersed composition (a) including a first set of microspheres modified with a biological/chemical probe and (b) containing an optical bar code generated from at least one colorant associated with the microspheres, and (II) a data region linked to said support outside of said active region having a stored digital file of a color map of the locations of said randomly dispersed first set of microspheres;
- exposing said active region of said system to an analyte;
- scanning exposed said active region by a monochrome microsphere map;
- reading with a digital reader said data region to load said digital file of said color microsphere map into said digital reader;
- processing said monochrome microsphere map and said color microsphere map to identify the identity of the exposed analyte(s).
Dependencies are inferred from the claim text.