Nucleic acid detection medium
OwnerMats Bo Johan Nilsson, Ulf Landegreninventors
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Abstract
An optimum reaction medium for performing nucleic acid detection and a method employing the medium are scribed.
Claims14
A medium for the efficient ligation of oligonucleotides hybridised to target RNA strands, the medium containing less than 50 mMol monovalent cations characterised in that the reaction medium concentration of ATP further contains a below or close to the Km for ligase adenylation.
A medium for the efficient ligation of oligonucleotides to target nucleic acid strands, characterised in that the medium includes 5-25 mM magnesium or manganese ions.
A method of detecting a target sequence of a target RNA characterized in that the method comprises the steps of:—
- i) providing a padlock probe for the target RNA sequence,
- ii) forming a hybrid of the padlock probe with the target RNA and circularising the padlock probe,
- iii) degrading the target RNA at or near the target sequence, and
- iv) affecting rolling circle replication of the padlock probe.
Dependencies are inferred from the claim text.