Integrin-linked kinase and its uses
AssigneeSunnybrook Health Sciences Centre
InventorsShoukat Dedhar, Greg Hannigan
Life of this patent
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Abstract
Methods for isolating ILK genes are provided.. The ILK nucleic acid compositions find use in identifying homologous or related proteins and the DNA sequences encoding such proteins; in producing compositions that modulate the expression or function of the protein; and in studying associated physiological pathways. In addition, modulation of the gene activity in vivo is used for prophylactic and therapeutic purposes, such as identification of cell type based on expression, and the like.
Claims21
An isolated nucleic acid encoding a mammalian ILK protein.
A purified polypeptide composition comprising at least 50 weight % of the protein present as a ILK protein or a fragment thereof.
A monoclonal antibody binding specifically to a ILK protein.
A non-human transgenic animal model for ILK gene function wherein said transgenic animal comprises an introduced alteration in an ILK gene.
A method of inhibiting the catalytic activity of mammalian ILK in a cell, the method comprising:
- decreasing the available level of [PtdIns (3,4,5) P3] in said cell;
- wherein the catalytic activity of said mammalian ILK is inhibited.
A method of screening for biologically active agents that modulate ILK function, the method comprising:
- combining a candidate biologically active agent with any one of:
- (a) a mammalian ILK polypeptide;
- (b) a cell comprising a nucleic acid encoding a mammalian ILK polypeptide;
- or
- (c) a non-human transgenic animal model for ILK gene function comprising one of:
- (i) a knockout of an ILK gene;
- (ii) an exogenous and stably transmitted mammalian ILK gene sequence;
- and
- determining the effect of said agent on ILK function.
A pharmaceutical composition comprising:
- a pharmaceutically acceptable carrier;
- and a biologically active agent that modulates ILK function.
Dependencies are inferred from the claim text.