Countercurrent chromatography separation of polar sulfonated compounds

A composition of greater than 99% pure sulfonated polar compound produced by a method for separating a quantity of a sulfonated polar compound from other compounds in a mixture using countercurrent chromatography, said method comprising:

• a) charging a cross axis countercurrent chromatographic centrifuge column with a first liquid;
• b) introducing a mixture comprising two or more sulfonated polar compounds into a combination of said first liquid and a second liquid, thereby producing a test mixture;
• c) introducing said test mixture into said column thus charged with said first liquid;
• and
• d) passing said second liquid through said column to elute said sulfonated polar compound from said column;
• wherein said first liquid comprises a solvent that is less polar than water and said second liquid is an aqueous solution.