Rapid evaluation of the ratio of biological molecules

A method for determining the ratio of oxidized cardiac troponin I to reduced cardiac troponin I in a patient sample, comprising:

• a) contacting the patient sample with
• i) a first antibody that specifically binds to both oxidized and reduced cardiac troponin I, wherein said oxidized and reduced cardiac troponin I bind to the first antibody in an amount proportional to their ratio in the sample, and
• ii) a second antibody that specifically binds to one of oxidized or reduced cardiac troponin I, whereby said first and second antibodies form a complex comprising one of said oxidized or reduced cardiac troponin I present in said sample, and do not form a complex comprising the other of said oxidized or reduced cardiac troponin I present in said sample;
• and
• b) generating a signal corresponding to the amount of said complex formed and relating said signal to said ratio.