Tolergenic antigen presenting cells and in treating immune-inflammatory conditions
OwnerJoan Stein-Streilein, J Wayne Streileininventors
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Abstract
The invention is directed to the preparation and use of tolerogenic antigen presenting cells as a method of down-regulating an immune response in a patient who has an established immune-inflammatory condition or who is at risk of suffering from an immune-inflammatory condition. The tolerogenic antigen presenting cells re-educate patient's own defense cells not only to suppress secretion of cytokines that generates an inflammatory response, but also to stimulate the secretion of immunosuppressive agents to ameliorate debilitating immune effects.
Claims60
A method of down-regulating an immune response in a patient, wherein said patient has an established immune-inflammatory condition, said method comprising the steps of:
- (a) providing said patient;
- (b) testing said patient for a response to an antigen;
- (c) upon a positive response to said antigen, collecting antigen presenting cells from said patient;
- (d) treating said antigen presenting cells with an inhibitory cytokine by incubation;
- (e) incubating said treated antigen presenting cells with said antigen, thereby generating tolerogenic antigen presenting cells;
- and
- (f) treating said patient with said tolerogenic antigen presenting cells, whereby said immune response is down-regulated in said patient.
A method of down-regulating an immune response in a patient, wherein said patient has an established immune-inflammatory condition or is believed to be at risk of suffering from an immune-inflammatory condition, said method comprising the steps of:
- (a) providing said patient;
- (b) isolating a population of antigen presenting cells from said patient;
- (c) treating said antigen presenting cells with an inhibitory cytokine by incubation;
- (d) incubating said treated antigen presenting cells with an antigen relevant to said condition, thereby generating tolerogenic antigen presenting cells;
- and
- (e) treating said patient with said tolerogenic antigen presenting cells.
A kit for making tolerogenic antigen presenting cells for treating an immune-inflammatory condition, said kit comprising:
- (a) an inhibitory cytokine;
- (b) an antigen relevant to said immune-inflammatory condition;
- and
- (c) instructions for use in making tolergenic antigen presenting cells for the treatment of said immune-inflammatory condition.
A kit for making tolerogenic antigen presenting cells for treating multiple sclerosis, said kit comprising:
- (a) transforming growth factor-β in a tolerogenic buffer;
- (b) myelin basic protein;
- and
- (c) instructions for use in making tolergenic antigen presenting cells for the treatment of multiple sclerosis.
An immunosuppressive agent for use during an immune-inflammatory condition, wherein said agent comprises a population of antigen presenting cells treated with an inhibitory cytokine and a relevant antigen, whereby said agent alters the reactivity of antigen presenting cells to become re-educated, thereby down-regulating an immune response in a patient with said immune-inflammatory condition.
Dependencies are inferred from the claim text.