Intravascular delivery of non-viral nucleic acid
OwnerJon A. Wolff, Sean D. Monahan +4inventors
InventorsJon A. Wolff, Sean D. Monahan, James E. Hagstrom, David B. Rozema, Vladimir G. Budker, Paul M. Slattum
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Abstract
A process is described for the delivery of a therapeutic polynucleotide to a tissue suffering from or potentially suffering from ischemia. The process comprises designing a polynucleotide for transfection. Then the polynucleotide is inserted into a mammalian vessel such as an artery or a vein. Prior to insertion, subsequent to insertion, or concurrent with insertion the volume of the tissue is increased such that the genetic material is delivered to the parenchymal cell.
Claims38
A process for delivering a protein or peptide to a muscle tissue of a patient for improving blood flow in the tissue comprising:
- a) injecting naked polynucleotides encoding the peptide or protein into a blood vessel lumen, in vivo;
- b) increasing extravascular volume in the muscle tissue;
- and, c) delivering the naked polynucleotides to extravascular muscle cells via the increased volume, wherein the polynucleotide is expressed.
A process delivering polynucleotides to a muscle tissue for enhancing blood flow in the tissue comprising:
- a) injecting naked polynucleotides into a blood vessel lumen, in vivo;
- b) increasing extravascular volume in the muscle tissue;
- and, c) delivering the naked polynucleotides to extravascular cells outside of the blood vessel via the increased volume.
Dependencies are inferred from the claim text.