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US 2004/0072783 A1Published application
Nucleozymes with endonuclease activity
OwnerRonald Breaker, Gail Emilssoninventors
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Abstract
The present invention relates to nucleozymes (DNA catalysts, DNA enzymes), methods of synthesis, and uses thereof.
Claims47
A nucleic acid molecule with endonuclease activity having the formula I:
- 3′-X-Z-Y-5′
- wherein, X and Y are independently oligonucleotides of lengths sufficient to stably interact with a target nucleic acid molecule, and Z is independently a nucleotide sequence comprising 5′-GATGCAGCTGGGGAGGCGTTT-3′ (SEQ ID NO 51).
A nucleic acid molecule with endonuclease activity having the formula II:
- 3′-X-W-V-R-Y-5′
- wherein, X and Y are independently oligonucleotides of length sufficient to stably interact with a target nucleic acid molecule, R is independently a nucleotide sequence comprising 5′-GGGGA-3′, V represents a nucleotide or non-nucleotide linker, which may be present or absent;
- W is independently an oligonucleotide comprising a nucleotide sequence selected from the group consisting of 5′-TGGGGAAGCACAGGGT-3′ (SEQ ID NO 52), 5′-TGGGGAAGCTCTGGGT-3′ (SEQ ID NO 53), 5′-TGGGGAAGCACAGGGT-3′ (SEQ ID NO 54), and 5′-TGGGGAAGCACAGGGT-3′(SEQ ID NO 55).
Dependencies are inferred from the claim text.