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OwnerRosamaria Ruggieri, Marinella Callow +1inventors
InventorsRosamaria Ruggieri, Marinella Callow, Paul W. Diaz
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The present invention relates to an isolated SRK polypeptide, biologically-active polypeptide fragments thereof, and nucleic acids which code for it. This polypeptide has various activities in regulating cell signaling and signal transduction pathways, including, e.g., a protein kinase activity; an autophosphorylating activity; a cell survival promoting activity; a HAX-1 binding activity; an apoptosis suppression activity; a MAPKK stimulatory activity; a transcription modulatory activity, and a SRK-specific immunogenic activity. The invention relates to all aspects of SRK, or homologs thereof, including assays for modulators, activators, ligands, etc. The invention also relates to a cytolic or soluble HAX-1 which produces apoptosis when expressed in cells.
An isolated human SRK polypeptide, or a biologically-active polypeptide fragment thereof.
An isolated SRK, or a biologically-active fragment thereof, which is coded for by the complement of a nucleic acid sequence which hybridizes under high stringency conditions to the DNA sequence set forth in FIG. 2 and which has at least 95% sequence identity to said DNA sequence.
An isolated nucleic acid comprising a nucleotide sequence coding for a full-length human SRK polypeptide, or a biologically-active polypeptide fragment thereof.
An isolated nucleic acid which hybridizes under high stringency conditions to the nucleic sequence set forth in FIG. 2, or a complement thereto, and which has at least 95% sequence identity to said nucleic sequence, or its complement.
An isolated nucleic acid consisting essentially of any continuous sequence of 12-100 base pairs, or a complement thereto, selected from the nucleotide sequence set forth in FIG. 2.
A method of detecting a protein kinase activity in a human SRK polypeptide, or a biologically-active polypeptide fragment thereof, comprising:
A method of identifying agents which modulate a MAPKK stimulatory activity of of a human SRK polypeptide, or a biologically-active polypeptide fragment thereof, comprising,
A method of identifying agents which modulate cellular tranformation mediated by Ras and SRK, comprising:
A method of identifying agents which modulate cellular transformation mediated by Ras and SRK, comprising:
A method of identifying agents that modulate the apoptosis suppression activity of human SRK polypeptide, or a biologically-active polypeptide fragment thereof, comprising:
A method of identifying agents that modulate the apoptosis suppression activity of human SRK polypeptide, or a biologically-active polypeptide fragment thereof, comprising:
An isolated antibody which is specific for a polypeptide sequence of human SRK.
A human cytosolic HAX-1 having an apoptotic activity.
A human cytosolic HAX-1, having an apoptotic activity, which is coded for by the complement of a nucleic acid sequence which hybridizes under high stringency conditions to the DNA sequence set forth in FIG. 7 and which has at least 95% sequence identity to said DNA sequence.
An isolated nucleic acid comprising a nucleotide sequence coding for a human cytosolic HAX-1.
Dependencies are inferred from the claim text.