Method of purifying lantibiotics
AssigneeImmuCell Corporation
InventorsRichard T. Coughlin, Joseph H. Crabb
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Abstract
Disclosed is a method for purifying a lantibiotic from a crude or partially purified solution containing the lantibiotic. In preferred embodiments, the lantibiotic is nisin, although the common structural features of lantibiotics dictate the effectiveness of the disclosed purification methods for other members of the lantibiotic genus. The method includes the step of forming an incubation mixture comprising the solution containing the lantibiotic and a proteolytic enzyme, and incubating the mixture under conditions optimized for selective proteolytic activity.
Claims13
A method for purifying a lantibiotic, the method comprising:
- a) providing a solution containing the lantibiotic to be purified;
- b) providing a proteolytic enzyme;
- and
- c) forming an incubation mixture comprising the solution containing the lantibiotic and the proteolytic enzyme, and incubating the mixture under conditions which are optimized for selective degradation of non-lantibiotic protein or polypeptide impurities, while leaving the lantibiotic substantially undigested.
Dependencies are inferred from the claim text.