Multiple cytokine protein complexes

3 The fusion protein of claim 1, wherein said C1 or said C2 comprise IL-2, IL-4 or GM-CSF.

A multifunctional protein complex comprising

• a first polypeptide chain defining an immunoglobulin region and a first portion of a first cytokine, and
• a second polypeptide chain defining a second cytokine and a second portion of the first cytokine.

A multifunctional protein complex comprising at least:

• a first fusion protein comprising a first cytokine fused to at least a portion of an immunoglobulin light chain, and
• a second fusion protein comprising a second, different cytokine fused to at least a portion of an immunoglobulin heavy chain.

A multifunctional fusion protein comprising a polypeptide chain defining a first cytokine (C1) and a second, different cytokine (C2), wherein said C2 when free has an circulating half life in vivo greater than about twice that of free C1, but when said C1 is linked to said C2 in said fusion protein said C1 has a circulating half life in vivo about the same as said C2 in said fusion protein.

A method of preparing a fusion protein comprising a first cytokine (C1), a second, different cytokine (C2), and a targeting moiety capable of targeting a preselected locus in a mammal, the method comprising the steps of:

• (a) expressing in a host cell a nucleic acid encoding a fusion protein comprising the C1, the C2 and the targeting moiety capable of targeting said fusion protein to the preselected locus when said fusion protein is administered to the mammal;
• and
• (b) harvesting said fusion protein.

A method of targeting a first cytokine (C1) and a second, different cytokine (C2) to a preselected locus in a mammal, the method comprising:

• administering to the mammal a multifunctional fusion protein comprising a C1, a C2, and an immunoglobulin region (Ig) capable of targeting said fusion protein to the preselected locus in the mammal.