Loading patent…
AssigneeThe General Hospital Corporation
InventorsBrian Seed, Janet Allen, Alejandro Aruffo, David Camerini, Leander Lauffer, Carmen Oquendo, David Simmons, Ivan Stamenkovic, Siegfried Stengelin, Martine Amiot
Estimating the term…
A simple and highly efficient method for cloning cDNAs from mammalian expression libraries based on transient expression in mammalian host cells has been discovered. Novel expression vectors allowing highly efficient construction of mammalian cDNA libraries are disclosed. The cloning method of the invention which has been used to clone genes for cell surface antigens of human lymphocytes, has general application in gene cloning. Cell surface antigens cloned according to the present invention have been purified, and the nucleotide and amino acid sequences determined. These antigens have diagnostic and therapeutic utility in immune-mediated infections in mammals, including humans.
A cloned cDNA segment encoding a cell surface antigen selected from the group consisting of CD1a, CD1b, CD1c, CD2, CD6, CD7, CD13, CD14, CD16, CD19, CD20, CD22, CD26, CD27, CD31, CD2w32a, CDw32b, CD33, CD34, CD36, CD37, CD38, CD39, CD40, CD43, and functional derivatives thereof.
A substantially pure protein selected from the group consisting of CD1a, CD1b, CD1c, CD2, CD6, CD7, CD13, CD14, CD16, CD19, CD20, CD22, CD26, CD27, CD31, CD2w32a, CDw32b, CD33, CD34, CD36, CD37, CD38, CD39, CD40, CD43, and functional derivatives thereof.
Substantially pure protein having the amino acid sequence shown in FIG. 2, or a functional derivative thereof.
Substantially pure human CD2 antigen, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 2, or a functional derivative thereof.
Substantially pure human CD7 antigen, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 8, or a functional derivative thereof.
Substantially pure protein having the amino acid sequence shown in FIG. 8, or a functional derivative thereof.
Substantially pure CDw32a antigen, or a functional derivative thereof.
Substantially pure CDw32b antigen, or a functional derivative thereof.
Substantially pure CD20 antigen, or a functional derivative thereof.
Substantially pure CD19 antigen, or a functional derivative thereof.
Substantially pure CD22 antigen, or a functional derivative thereof.
Substantially pure CD40 antigen, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 15, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 16, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 13, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 12, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in Table 4, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in FIG. 17, or a functional derivative thereof.
Substantially pure protein having the amino acid sequence shown in FIG. 10, or a functional derivative thereof.
Substantially pure protein having the amino acid sequence shown in Table 4, or a functional derivative thereof.
Substantially pure CD27 antigen, or a functional derivative thereof.
Substantially pure cDNA comprising the nucleotide sequence shown in Table 5, or a functional derivative thereof.
Substantially pure protein having the amino acid sequence shown in Table 5, or a functional derivative thereof.
Dependencies are inferred from the claim text.