Methods and products for manipulating hematopoietic stem cells
OwnerDavid T. Scadden, Byeong-Chel Leeinventors
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Abstract
The invention relates to methods for manipulating hematopoietic stem cells and related products. In one aspect the invention relates to the use of stem cell G-protein coupled receptor (SC-GPR) related compositions to identify bone marrow derived hematopoietic stem cells, to enhance mobilization of hematopoietic stem cells, to improve the efficiency of targeting cells to the bone marrow and/or to modulate hematopoietic cell function.
Claims53
A method for identifying a hematopoietic stem cell, comprising:
- identifying the presence or absence of a stem cell G-protein coupled receptor (SC-GPR) on a putative hematopoietic stem cell, the presence of the SC-GPR being indicative of a hematopoietic stem cell.
A method for isolating a hematopoietic stem cell, comprising:
- contacting a sample containing a hematopoietic stem cell with a SC-GPR binding agent to isolate the hematopoietic stem cell from the sample, wherein the SC-GPR binding agent is not N-acetylglucosamine.
A kit, comprising:
- a stem cell G-protein coupled receptor (SC-GPR) binding agent, and
- instructions for contacting a hematopoietic stem cell with the SC-GPR binding agent to identify or isolate the hematopoietic stem cell.
A SC-GPR-transduced hematopoietic cell.
A method for supplementing bone marrow, comprising:
- administering to a subject a SC-GPR enriched hematopoietic cell population to supplement the bone marrow of the subject.
A method for enhancing mobilization of hematopoietic stem cells, comprising:
- administering to a subject a SC-GPR inhibitor to enhance mobilization of hematopoietic stem cells in the subject.
A method for modulating hematopoietic cell function, comprising:
- contacting a hematopoietic cell with a SC-GPR activator or a SC-GPR inhibitor to modulate hematopoietic cell function.
A method for inducing hematopoietic stem cell quiescence, comprising:
- contacting a hematopoietic stem cell with a SC-GPR activator to induce quiescence of the hematopoietic stem cell.
A method for in vitro culture of hematopoietic cells to produce differentiated cells of non-hematopoietic lineage, comprising:
- contacting a hematopoietic cell with a SC-GPR molecule under conditions sufficient to confer hematopoietic stem cell characteristics to the hematopoietic cell, and
- culturing the hematopoietic cell having stem cell characteristics in an environment that promotes hematopoietic cell differentiation, under conditions and for a period of time to produce differentiated cells of non-hematopoietic lineage.
Dependencies are inferred from the claim text.