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AssigneeLexicon Genetics Incorporated
InventorsArthur Sands, Glenn Friedrich, Brian Zambrowicz, Allan Bradley
Estimating the term…
Methods and vectors (both DNA and retroviral) are provided for the construction of a Library of mutated cells. The Library will preferably contain mutations in essentially all genes present in the genome of the cells. The nature of the Library and the vectors allow for methods of screening for mutations in specific genes, and for gathering nucleotide sequence data from each mutated gene to provide a database of tagged gene sequences. Such a database provides a means to access the individual mutant cell clones contained in the Library. The invention includes the described Library, methods of making the same, and vectors used to construct the Library. Methods are also provided for accessing individual parts of the Library either by sequence or by pooling and screening. The invention also provides for the generation of non-human transgenic animals which are mutant for specific genes as isolated and generated from the cells of the Library.
A library of cultured eucaryotic cells made by a process comprising the steps of:
A vector for replacing the 3′ end of an animal cell transcript with a foreign-exon, comprising:
A vector for inserting foreign exons internal to animal cell transcripts, comprising:
A vector for attaching a foreign exon upstream from the 3′ end of an animal cell transcript, comprising:
Dependencies are inferred from the claim text.