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US 2004/0072208 A1Published application
Surface acoustic wave sensors and method for detecting target analytes
OwnerPeter Warthoe, Soerensen Ibeninventors
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Abstract
The present invention relates generally to methods and compositions for analyzing binding molecules including proteins and nucleic acid molecules. In addition the invention relates to the use of surface acoustic wave sensors that rely on non-fluorescent detection system consisting of a sensor using propagation of surface acoustic waves for detection of a wide variety of biological-based assays.
Claims10
A method for determining the presence or absence of a target nucleic acid in a test sample comprising:
- contacting a target nucleic acid comprising first and second adjacent regions with a surface acoustic wave sensor comprising a surface having an immobilized probe nucleic acid which hybridizes to said first region of said test nucleic acid to form a hybridization complex, wherein said first region of said target nucleic acid is double stranded and said adjacent second region of said target nucleic acid is single stranded in said hybridization complex;
- extending the probe nucleic acid in said hybridization complex using said second region in said test nucleic acid as template;
- applying an input signal to an input transducer to generate a surface acoustic wave within said surface acoustic wave sensor;
- receiving said surface acoustic wave at an output transducer;
- generating an electronic output signal;
- and
- measuring a parameter of said output signal which provides an indication of whether or not said target nucleic acid is present in said test sample.
4 more elements
Dependencies are inferred from the claim text.