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US 2004/0072206 A1Published application
Method for identifying modulators of transcription
OwnerJeffrey Errington, Helena Barbara Thomaidesinventors
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OwnerJeffrey Errington, Helena Barbara Thomaidesinventors
Estimating the term…
A method for identifying a modulator of RNA polymerase (RNAP) comprises providing a host cell which expresses a first RNAP and has a first and second polynucleotide construct. The first polynucleotide construct comprises a first promoter operably linked to a first gene wherein the first gene is transcribed by the first RNAP. The second polynucleotide construct comprises a second promoter operably linked to a second gene which is a reporter gene and wherein the second reporter gene is transcribed by a second RNAP. The host cell is provided with a source of the second RNAP. A test substance is contacted with the host cell under conditions that would permit the expression of the first and second genes in the absence of the test substance. It can be determined thereby whether the test substance modulates RNAP.
A method for identifying a modulator of RNA polymerase (RNAP) comprising:
A method according to any one of the preceding claims wherein the second RNAP is derived from an heterologous organism to the first RNAP.
A method according to any one of the preceding claims wherein the second RNAP comprises a viral RNAP.
A method according to any one of the preceding claims wherein the host cell comprises a bacterial cell.
A method according to any one of the preceding claim wherein the first and/or second promoters are inducible promoters.
A method according to any one of the preceding claims comprising determining whether the test substance modulates only one of the first or second RNAP activity.
Dependencies are inferred from the claim text.