Purification of primer extension products

A method for purifying a primer extension product, said method comprising:

• (a) extending a primer that comprises a string of arylboronic acid moieties using a primer extension reaction to form primer extension products;
• (b) contacting the primer extension products of (a) with a solid support having attached thereto an arylboronic acid complexing moiety to form a complex comprising the primer extension products and the solid support;
• and
• (c) separating the complex of (b) from the liquid phase of the primer extension reaction.

A method for isolating a nucleic acid, the method comprising:

• (a) contacting a sample comprising the nucleic acid with a probe to form a nucleic acid hybrid, wherein the probe comprises a string of arylboronic acid moieties;
• (b) contacting the nucleic acid hybrid of (a) with a solid support having attached thereto a arylboronic acid complexing moiety to form a complex comprising the nucleic acid hybrid and the solid support;
• and
• (c) separating the complex of (b) from the sample.

A method for purifying a nucleic acid sequencing reaction product, the method comprising:

• (a) hybridizing a primer that comprises a string of arylboronic acid moieties to a nucleic acid template to form a template-primer hybrid;
• (b) extending the primer by contacting the hybrid with a polymerase in a reaction mixture comprising deoxynucleotides and dideoxynucleotides to form a primer extension product;
• (c) contacting the primer extension product of (b) with a solid support having attached thereto a arylboronic acid complexing moiety to form a complex comprising the primer extension product and the solid support;
• and
• (d) separating the complex of (c) from the reaction mixture.