Modified nucleic acid probes
AssigneeAffymetrix, Inc.
InventorsGlenn Hugh McGall, Charles Garrett Miyada, Maureen T. Cronin, Jennifer Dee Tan, Mark S. Chee
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Abstract
Oligonucleotide analogue arrays attached to solid substrates and methods related to the use thereof are provided. The oligonucleotide analogues hybridize to nucleic acids with either higher or lower specificity than corresponding unmodified oligonucleotides. Target nucleic acids which comprise nucleotide analogues are bound to oligonucleotide and oligonucleotide analogue arrays.
Claims36
A composition comprising an array of oligonucleotide analogues attached to a solid substrate.
A composition comprising an oligonucleotide analogue array synthesized on a solid substrate, wherein said synthesis is performed by light-directed chemical coupling.
A composition comprising an oligonucleotide analogue array synthesized on a solid substrate, wherein said synthesis is performed by flowing oligonucleotide analogue reagents over predetermined regions of the solid substrate.
A method of improving the hybridization of a nucleic acid to an olgionucleotide array, comprising incorporating a base selected from the group consisting of 7-deazaguanosine, 2-aminopurine, 8-aza-7-deazaguanosine, 1H-purine, and hypoxanthine into the oligonucleotides of the array.
A method of determining if a target molecule is complementary to a probe, comprising the steps of:
- (a) synthesizing an oligonucleotide analogue array on a solid substrate;
- (b) exposing said oligonucleotide analogue array on said solid substrate to a target nucleic acid;
- and
- (c) determining whether an oligonucleotide analogue member of said oligonucleotide analogue array binds to said target oligonucleotide.
A composition comprising an array of oligonucleotide probes hybridized to a target nucleic acid, which target nucleic acid comprises a nucleotide analogue.
A method of detecting a target nucleic acid, comprising enzymatically copying the target nucleic acid using nucleotides which comprise a nucleotide analogue, thereby producing a nucleic acid analogue amplicon, and hybridizing the nucleic acid amplicon to an oligonucleotide array.
Dependencies are inferred from the claim text.