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US 2004/0072187 A1Published application
Novel mammalian receptor genes and uses
AssigneeOregon Health & Science University
InventorsJames Bunzow, David K. Grandy, Mark Sonders
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AssigneeOregon Health & Science University
InventorsJames Bunzow, David K. Grandy, Mark Sonders
Estimating the term…
The present invention relates to novel mammalian biogenic amine receptor proteins and genes that encode such proteins. The invention is directed toward the isolation and characterization of mammalian trace amine receptor proteins. The invention specifically provides isolated complementary DNA copies of mRNA corresponding to rat and human homologues of a mammalian trace amine receptor gene. Also provided are recombinant expression constructs capable of expressing the mammalian trace amine receptor genes of the invention in cultures of transformed prokaryotic and eukaryotic cells, as well as such cultures of transformed cells that synthesize the mammalian trace amine receptor proteins encoded therein. The invention also provides methods for screening compounds in vitro that are capable of binding to the mammalian trace amine receptor proteins of the invention, and further characterizing the binding properties of such compounds and functional consequences thereof in comparison with known trace amine receptor agonists and antagonists. Improved methods of pharmacological screening are provided thereby.
A nucleic acid comprising a nucleotide sequence encoding a mammalian biogenic amine receptor.
A homogeneous composition of a mammalian trace amine receptor or derivative thereof having a molecular weight of about 39 kilodaltons and an amino acid sequence identified by (SEQ ID No.:
A homogeneous composition of a mammalian trace amine receptor or derivative thereof having a molecular weight of about 38 kilodaltons and an amino acid sequence identified by (SEQ ID No.:
A nucleic acid hybridization probe comprising a nucleotide sequence identified by Seq ID No. 1.
A nucleic acid hybridization probe comprising a nucleotide sequence identified by Seq ID No. 3.
A nucleic acid comprising a nucleotide sequence encoding a mammalian trace amine receptor that hybridizes to a nucleic acid having a nucleotide sequence identified by Seq. ID No. 1, under conditions of 37° C. in a buffer comprising 50% formamide, 1% sodium dodecyl sulfate, 5×SSC, 50 μg/mL denatured salmon sperm DNA, and 5× P-buffer comprising 0.25M Tris, pH 7.5, 0.5% sodium pyrophosphate, 0.5%. SDS, 1% bovine serum albumin, 1% polyvinylpyrrolidone and 1% Ficoll.
A nucleic acid comprising a nucleotide sequence encoding a mammalian trace amine receptor that hybrids to a nucleic acid having a nucleotide sequence identified by Seq. ID No. 3, under conditions of 37° C in a buffer comprising 50% formamide, 1% sodium dodecyl sulfate, 5×SSC, 50 μg/mL denatured salmon sperm DNA, and 5× P-buffer comprising 0.25M Tris, pH 7.5, 0.5% sodium pyroposphate, 0.5% SDS, 1% bovine serum albumin, 1% polyvinylpyrrolidone and 1% Ficoll.
A cell membrane preparation comprising a mammalian trace amine receptor or derivative thereof having a molecular weight of about 39 kilodaltons and an amino acid sequence identified by SEQ ID No.:
A cell membrane preparation comprising a mammalian trace amine receptor or derivative thereof having a:
A cytosolic preparation comprising a mammalian trace amine receptor or derivative thereof having a molecular weight of about 39 kilodaltons and an ammo acid sequence identified by SEQ ID No.:
A cytosolic preparation comprising a mammalian trace amine receptor or derivative thereof having a molecular weight of about 38 kilodaltons and an amino acid sequence identified by SEQ ID No.:
Dependencies are inferred from the claim text.