Adhesive protein and uses thereof
OwnerPer-Erik Olsson, Jana Jaas +3inventors
InventorsPer-Erik Olsson, Jana Jaas, Iwan Jones, Staffan Jakobsson, Bertil Borg
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Abstract
An adhesive protein, present inter alia in the stickleback nest building glue has now been isolated and purified. Its amino acid sequence and corresponding coding polynucleotide sequences are isolated and sequenced. The protein finds both medical and technical uses, whereas both the protein and the polynucleotide sequence can be used in analyses, determining the presence and influence of androgenic substances, for example androgenic pollutants, present in the aquatic environment.
Claims34
A substantially purified adhesive protein comprising the amino acid sequence of SEQ ID NO:
- 6 or fragments thereof.
A substantially purified adhesive protein comprising the amino acid sequence of SEQ ID NO:
- 3 or fragments thereof.
A substantially purified adhesive protein comprising the amino acid sequence of SEQ ID NO:
- 7 or fragments thereof.
An isolated and purified polynucleotide sequence comprising SEQ ID NO:
- 5 or functionally equivalent variants thereof, said sequence and variants coding an adhesive protein.
An isolated and purified polynucleotide sequence comprising SEQ ID NO:
- 2 or functionally equivalent variants thereof, said sequence and variants coding an adhesive protein.
An isolated and purified polynucleotide sequence comprising SEQ ID NO:
- 8 or functionally equivalent variants thereof, said sequence and variants coding an adhesive protein.
An isolated and purified polynucleotide sequence comprising SEQ ID NO:
- 4 or functionally equivalent variants thereof, said sequence and variants coding an adhesive protein.
An isolated and purified polynucleotide sequence comprising SEQ ID NO:
- 1 or functionally equivalent variants thereof, said sequence and variants coding an adhesive protein.
An isolated and purified polynucleotide sequence comprising SEQ ID NO:
- 9 or functionally equivalent variants thereof, said sequence and variants coding an adhesive protein.
A method for producing a polypeptide comprising the amino acid sequence of SEQ ID NO:
- 6 or fragments thereof, the method comprising the steps of:
- culturing the host cell of claim 18 under conditions suitable for the expression of the polypeptide;
- and
- recovering the polypeptide from the host cell culture.
A method for the detection of androgenic compounds in biological systems, characterized in that said method comprises the following steps:
- collection of samples of kidney or urinary bladder from sticklebacks,
- extraction of RNA,
- separation of RNA on agarose gel,
- transfer to nylon membrane,
- prehybridisation followed by hybridisation with labelled spiggin cDNA or cRNA,
- incubation and washing, and
- detection of specific signal.
Dependencies are inferred from the claim text.