Dna binding peptide domains and a method for providing domains of this type
OwnerAndreas Kappel, Norbert Windhab +3inventors
InventorsAndreas Kappel, Norbert Windhab, Thomas Wagner, Stefan Kienle, Karsten Kuhn
Life of this patent
Estimating the term…
Abstract
The present invention relates to peptidic domains which can be readily synthesized and which can specifically recognize and bind nucleic acid sequences and to a method for finding and providing peptide domains which bind specifically to DNA and biomorphic factors derived therefrom, in particular transcription factors and repressors.
Claims24
A biomorphic peptide comprising an amino acid sequence according to Seq. ID No. 1 or having an amino acid sequence which is at least 75% homologous to Seq. ID No. 1, which peptide can specifically bind to a nucleic acid sequences, with the exception of the amino acid sequences having the amino acids N in position 10, A in position 13, A or L in position 14, and S in position 17.
The biomorphic peptide as claimed in any of the preceding claims, characterized in that at least two peptides selected from the group according to Seq. ID No. 1 or having an amino acid sequence which is at least 75% homologous to Seq. ID No. 1 are linked via a linker.
The biomorphic peptide as claimed in any of the preceding claims, comprising an amino acid sequence according to Seq. ID No. 2.
The biomorphic peptide as claimed in any of the preceding claims, comprising an amino acid sequence according to Seq. ID No. 1, an amino acid sequence which is at least 75% homologous to Seq. ID No. 1 or to Seq. ID No. 2 and nuclear transport signal domains, transcription-activating domains or transcription-inhibiting domains.
The use of biomorphic peptides comprising an amino acid sequence according to Seq. ID No. 1, an amino acid sequence which is at least 75% homologous to Seq. ID No. 1 or to Seq. ID No. 2 for producing pharmaceuticals for the treatment of gene regulation disorders.
A library comprising deoxyribonucleic acids which comprise a nucleic acid sequence according to Seq. ID No. 3 or a nucleic acid sequence coding for an amino acid sequence which is at least 75% homologous to Seq. ID No. 1 and a region encoding a transcription-activating domain.
A biomorphic peptide comprising an amino acid sequence according to Seq. ID No. 1 or to a structural variant, characterized in that the amino acid at position X-1 is G, C, I, D, N, S, P, H or R, the amino acid at position X-2 is V, A, N, M, L, E, G, P, S, T, Y or R, the amino acid at position X-3 is V, S, G, T, L, M, A, I, E, N, C, Q, Y or D, the amino acid at position X-4 is G, C, I, H, S, T, M, Q, A, L, W, V, D, K or R.
Dependencies are inferred from the claim text.